Nanoparticle In Vitro Toxicity Panel

 

  HemoGenix® is a Compliant Contract Research Service Supplier through Scientist.com

 

HemoGenix Contract Research Services

 

For more information on nanotoxicity services, please contact HemoGenix® at contractresearch@hemogenix.com or call (719) 264-6250. These services are also available through Science Exchange or Scientist.com.

 

The National Cancer Institute (NCI) Nanoparticle Characterization Laboratory (NCL) has established an assay cascade for testing nanoparticles used for clinical purposes.

 

The Assay Cascade proposed by the NCI NCL consists of 4 catagories of testing:

  • Sterility and Endotoxin
  • Physiochemical Characterization
  • In Vitro Characterization
  • In Vivo Characterization

Many of the recommended in vitro tests for nanoparticles now have far superior alternatives, many of which only HemoGenix® Contract Research Services can provide. 

 

 

The majority of the NCL recommended assays for in vitro toxicity testing of nanoparticles are based on either animal cells or transformed cell lines derived from human or other species. These do not provide the best extrapolation to the human situation especially when clinical trials are started. If fact, it is possible that using animal cells and cell lines will not predict the human response. 

 

For this reason, HemoGenix® not only provides the NCL recommended assay, but also it's own alternative assays using either the same target cells or human target cells.

 

 

NCL Recommended In Vitro Nanoparticle Characterization Assays and Alternatives
NCL Recommended Assay NCL Protocol No. HemoGenix® Alternative Assay
Mouse granulocyte-macrophage (GM) colony-forming cell (CFU) assay ITA-3 HALO®-Tox HT for multiple stem and progenitor cell populations for primary bone marrow from human, primate, dog, rat or mouse
Leukocyte proliferation assay ITA-6 ImmunoGlo™-Tox HT from human peripheral blood and/or human peripheral blood for T- or B-cells and subpopulations.
Nitric oxide (NO) production by RAW264.7 macrophage cell line ITA-7 Nitric oxide production from human, primary macrophage and cell lines e.g. RAW264.7

Chemotaxis assay using the human

HL-60 cell line

 ITA-8 Calcein-AM real-time fluorescence chemotaxis using HL-60 and primary, human peripheral blood or bone marrow cells
Zymosan phagocytosis assay using luminol on the HL-60 cell line ITA-9 Zymosan phagocytosis using primary, human peripheral blood or bone marrow cells or cell lines, e.g. HL-60
Analysis of IL-8, IL-1b, TNF alpha, IGN gamma by normal human peripheral blood mononuclear cells by ELISA ITA-10, 22, 23, 24, 25 Detection of cytokines and other growth factors from primary, human peripheral blood or bone marrow using multiplexing LUMINEX Technology

Effects on peripheral blood monocyte-derived dendritic cell maturation using flow cytometry

 ITA-14 Effects on peripheral blood monocyte-derived dendritic cell maturation using flow cytometry

Oxidative stress on Hep-G2 hepatocyte using the glutathione assay

 GTA-3 Oxidative stress using also primary hepatocytes, iPS-derived hepatocytes or other hepatocyte target cells (e.g. Hep-G2) using a glutathione assay

Lipid peroxidation assay on Hep-G2 cells

GTA-4

 Lipid peroxidation assay using using primary hepatocytes, iPS-derived hepatocytes or hepatocyte cell lines e.g. Hep-G2
Reactive oxygen species (ROS) assay on Hep-G2 hepatocytes GTA-7

Reactive oxygen species (ROS) using primary, hepatocytes, iPS-derived hepatocytes or hepatocyte cells lines (e.g. Hep-G2)

Kidney cytotoxicty assay using MTT and LDH assays on LLC-PK1 kidney cells

 GTA-1 Kidney cytotoxicity assay using primary, human kidney proximal tubule cells or kidney cell lines (e.g. LLC-Pk1) using RenalGlo™-Tox HT or MTT metabolic viability assays and LDH assays 

Hep-G2 hepatocyte cytotoxicity assay using MTT and LDH assays

 GTA-2 Hepatocyte cytotoxicity using primary, human hepatocytes, iPS-derived hepatocytes or human, hepatocyte cell lines (e.g. Hep-G2) using HepatoGlo™-Tox HT or other metabolic viability assay and LDH fluorescence assays
Kidney apoptosis assay for caspase 3 using LLC-PK1 cells  GTA-5 Caspase 3 apoptosis assay using primary, human proximal tubule kidney cells or kidney cell lines (e.g. LLC-PK1) 

Caspase 3  and 3/7 apoptosis assays on Hep-G2 cells

 GTA-6, GTA-14 Caspase 3 and 3/7 apoptosis assays using primary, human hepatocytes, iPS-derived hepatocytes or hepatocyte cell lines (e.g. Hep-G2)

 

Download the Nanotoxicity In Vitro Testing Panel Flyer

 

You can perform nanoparticle toxicity testing for hematotoxicity, immunotoxicity, hepatotoxicity, neurotoxicity and toxicity to numerous other primary tissues and cell lines in-house using assay kits sold by Preferred Cell Systems™. Please click on the links below to take you to the assay kit page on the Preferred Cell Systems website.

 

HALO®-Tox HT

ImmunoGlo™-Tox HT

HepatoGlo™-Tox HT

NeuroGlo™-Tox HT

STEMGlo™-Tox HT